AP Bio unit 6 eukaryotic gene expression cheat sheet
Study essential concepts of eukaryotic gene expression, including transcription, translation, regulation, and related biochemical processes for AP Biology.
Quiz(32 questions)
1. What is the primary function of RNA polymerase in transcription?
Terms in this Study Set(32)
Transcription and RNA Processing(16)
What is transcription?
The process of synthesizing RNA from a DNA template.
Key enzyme in transcription?
RNA polymerase catalyzes the synthesis of RNA.
True or False: Transcription occurs in the cytoplasm.
False: Transcription occurs in the nucleus of eukaryotic cells.
What are the three stages of transcription?
- Initiation - Elongation - Termination
Initiation of transcription involves which promoter?
The TATA box is a key promoter region.
Fill in the blank: RNA polymerase adds nucleotides in the _____ direction.
5' to 3' direction.
What is the role of transcription factors?
They help RNA polymerase bind to the promoter.
Post-transcriptional modification: What is capping?
Addition of a 5' cap for stability and recognition.
What is polyadenylation?
Addition of a poly-A tail to the 3' end of mRNA.
Key difference between pre-mRNA and mature mRNA?
Pre-mRNA contains introns; mature mRNA has introns spliced out.
True or False: Introns are coding sequences.
False: Introns are non-coding sequences in pre-mRNA.
What is splicing?
The removal of introns and joining of exons in pre-mRNA.
Mechanism: How does RNA polymerase know where to start?
It binds to the promoter region marked by transcription factors.
What are exons?
Coding sequences that remain in the mRNA after splicing.
What is the significance of the 5' cap?
It protects mRNA from degradation and aids in ribosome binding.
Cause → Effect: What happens without the poly-A tail?
mRNA is rapidly degraded in the cytoplasm.
Translation and Post-Translational Modifications(16)
What is translation?
The process of synthesizing proteins from mRNA templates in ribosomes.
List the stages of translation.
- Initiation - Elongation - Termination
What role do ribosomes play in translation?
Ribosomes facilitate the binding of tRNA to mRNA and catalyze peptide bond formation.
True or False: Translation occurs in the nucleus.
False. Translation occurs in the cytoplasm.
What is the function of tRNA?
tRNA transports amino acids to the ribosome and matches them to the mRNA codons.
Complete the statement: The start codon is...
AUG, which codes for methionine.
Define post-translational modification.
Chemical modifications made to a protein after translation, affecting its function and activity.
List examples of post-translational modifications.
- Phosphorylation - Glycosylation - Ubiquitination
What is phosphorylation?
The addition of phosphate groups to a protein, which can alter its activity.
What is ubiquitination?
Tagging of proteins for degradation by the proteasome, regulating protein levels.
True or False: All proteins undergo post-translational modifications.
False. Not all proteins are modified; some remain in their primary form.
How do signal peptides function?
They direct the protein to specific cellular locations, like the ER for secretion.
What is the role of chaperone proteins?
Chaperones assist in proper folding of proteins, preventing aggregation.
Fill in the blank: Elongation in translation involves the addition of ________.
amino acids to the growing polypeptide chain.
What is the effect of mutations on translation?
Mutations can lead to the incorporation of incorrect amino acids, potentially altering protein function.
Compare prokaryotic and eukaryotic translation.
Eukaryotic translation occurs in the cytoplasm, while prokaryotic occurs in the cytoplasm simultaneously with transcription.
Questions in this Study Set(32)
1. What is the primary function of RNA polymerase in transcription?
2. What is the primary function of ribosomal RNA (rRNA) in translation?
3. During which stage of transcription does RNA polymerase synthesize the RNA strand?
4. During which stage of translation does the ribosome assemble around the mRNA?
5. What is the role of transcription factors in eukaryotic cells?
6. Which of the following correctly describes the role of the A site in the ribosome?
7. Which region is critical for the initiation of transcription in eukaryotes?
8. What is the consequence of a frameshift mutation during translation?
9. Which of the following statements about introns is TRUE?
10. Which of the following is NOT a type of post-translational modification?
11. What modification does the 3' end of mRNA undergo?
12. How does the presence of a signal peptide affect protein synthesis?
13. Which process is responsible for removing introns from pre-mRNA?
14. In eukaryotic cells, where does translation take place?
15. What would happen if the 5' cap is not added to mRNA?
16. Which of the following post-translational modifications can regulate protein activity?
17. Which of the following is NOT a stage of transcription?
18. Which statement best describes the difference between eukaryotic and prokaryotic translation?
19. Fill in the blank: RNA polymerase adds nucleotides in the _____ direction.
20. What does the term 'peptidyl transferase' refer to?
21. Which statement best describes exons?
22. What is the purpose of chaperone proteins in post-translational modifications?
23. What initiates transcription in eukaryotic cells?
24. Which codon is recognized as the start codon in eukaryotes?
25. Why is polyadenylation important for mRNA stability?
26. What is the role of elongation factors during translation?
27. True or False: Transcription occurs in the cytoplasm of eukaryotic cells.
28. Which post-translational modification is commonly associated with signaling pathways?
29. What is the primary role of the 5' cap added during RNA processing?
30. What happens during the termination stage of translation?
31. Which of the following statements about RNA splicing is FALSE?
32. What is the role of the E site in the ribosome?
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