AP Bio unit 6 eukaryotic gene expression cheat sheet

Study essential concepts of eukaryotic gene expression, including transcription, translation, regulation, and related biochemical processes for AP Biology.

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What is transcription?

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The process of synthesizing RNA from a DNA template.

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Quiz(32 questions)

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1. What is the primary function of RNA polymerase in transcription?

Terms in this Study Set(32)

Transcription and RNA Processing(16)

What is transcription?

The process of synthesizing RNA from a DNA template.

Key enzyme in transcription?

RNA polymerase catalyzes the synthesis of RNA.

True or False: Transcription occurs in the cytoplasm.

False: Transcription occurs in the nucleus of eukaryotic cells.

What are the three stages of transcription?

- Initiation - Elongation - Termination

Initiation of transcription involves which promoter?

The TATA box is a key promoter region.

Fill in the blank: RNA polymerase adds nucleotides in the _____ direction.

5' to 3' direction.

What is the role of transcription factors?

They help RNA polymerase bind to the promoter.

Post-transcriptional modification: What is capping?

Addition of a 5' cap for stability and recognition.

What is polyadenylation?

Addition of a poly-A tail to the 3' end of mRNA.

Key difference between pre-mRNA and mature mRNA?

Pre-mRNA contains introns; mature mRNA has introns spliced out.

True or False: Introns are coding sequences.

False: Introns are non-coding sequences in pre-mRNA.

What is splicing?

The removal of introns and joining of exons in pre-mRNA.

Mechanism: How does RNA polymerase know where to start?

It binds to the promoter region marked by transcription factors.

What are exons?

Coding sequences that remain in the mRNA after splicing.

What is the significance of the 5' cap?

It protects mRNA from degradation and aids in ribosome binding.

Cause → Effect: What happens without the poly-A tail?

mRNA is rapidly degraded in the cytoplasm.

Translation and Post-Translational Modifications(16)

What is translation?

The process of synthesizing proteins from mRNA templates in ribosomes.

List the stages of translation.

- Initiation - Elongation - Termination

What role do ribosomes play in translation?

Ribosomes facilitate the binding of tRNA to mRNA and catalyze peptide bond formation.

True or False: Translation occurs in the nucleus.

False. Translation occurs in the cytoplasm.

What is the function of tRNA?

tRNA transports amino acids to the ribosome and matches them to the mRNA codons.

Complete the statement: The start codon is...

AUG, which codes for methionine.

Define post-translational modification.

Chemical modifications made to a protein after translation, affecting its function and activity.

List examples of post-translational modifications.

- Phosphorylation - Glycosylation - Ubiquitination

What is phosphorylation?

The addition of phosphate groups to a protein, which can alter its activity.

What is ubiquitination?

Tagging of proteins for degradation by the proteasome, regulating protein levels.

True or False: All proteins undergo post-translational modifications.

False. Not all proteins are modified; some remain in their primary form.

How do signal peptides function?

They direct the protein to specific cellular locations, like the ER for secretion.

What is the role of chaperone proteins?

Chaperones assist in proper folding of proteins, preventing aggregation.

Fill in the blank: Elongation in translation involves the addition of ________.

amino acids to the growing polypeptide chain.

What is the effect of mutations on translation?

Mutations can lead to the incorporation of incorrect amino acids, potentially altering protein function.

Compare prokaryotic and eukaryotic translation.

Eukaryotic translation occurs in the cytoplasm, while prokaryotic occurs in the cytoplasm simultaneously with transcription.

Questions in this Study Set(32)

1. What is the primary function of RNA polymerase in transcription?

A.Synthesizes RNA from a DNA template
B.Synthesizes proteins from RNA
C.Splices introns from pre-mRNA
D.Adds a 5' cap to mRNA

2. What is the primary function of ribosomal RNA (rRNA) in translation?

A.To form the core of ribosome structure and catalyze peptide bond formation
B.To transport amino acids to the ribosome
C.To encode the proteins being synthesized
D.To initiate the transcription process

3. During which stage of transcription does RNA polymerase synthesize the RNA strand?

A.Initiation
B.Elongation
C.Termination
D.Capping

4. During which stage of translation does the ribosome assemble around the mRNA?

A.Termination
B.Initiation
C.Elongation
D.Recycling

5. What is the role of transcription factors in eukaryotic cells?

A.Assist RNA polymerase in binding to the promoter
B.Degrade mRNA after translation
C.Add poly-A tails to mRNA
D.Remove exons from pre-mRNA

6. Which of the following correctly describes the role of the A site in the ribosome?

A.It holds the tRNA carrying the growing polypeptide chain
B.It is where the newly arrived tRNA with the next amino acid binds
C.It is responsible for the release of the polypeptide chain
D.It catalyzes the formation of peptide bonds

7. Which region is critical for the initiation of transcription in eukaryotes?

A.TATA box
B.Shine-Dalgarno sequence
C.Origin of replication
D.Anti-sense strand

8. What is the consequence of a frameshift mutation during translation?

A.It may lead to a nonfunctional protein due to altered amino acid sequence
B.It has no effect on the amino acid sequence
C.It only affects the last amino acid of the protein
D.It can result in a longer protein with additional amino acids

9. Which of the following statements about introns is TRUE?

A.They are coding sequences in pre-mRNA
B.They are non-coding sequences in pre-mRNA
C.They are removed during translation
D.They are added during transcription

10. Which of the following is NOT a type of post-translational modification?

A.Phosphorylation
B.Translation
C.Glycosylation
D.Ubiquitination

11. What modification does the 3' end of mRNA undergo?

A.Addition of a 5' cap
B.Addition of a poly-A tail
C.Splicing of introns
D.Formation of ribosomes

12. How does the presence of a signal peptide affect protein synthesis?

A.It prevents the protein from folding
B.It directs the protein to the endoplasmic reticulum for secretion
C.It enhances the binding of mRNA to ribosomes
D.It initiates the transcription process

13. Which process is responsible for removing introns from pre-mRNA?

A.Capping
B.Splicing
C.Polyadenylation
D.Transcription

14. In eukaryotic cells, where does translation take place?

A.In the nucleus
B.In the mitochondria
C.In the cytoplasm
D.In the Golgi apparatus

15. What would happen if the 5' cap is not added to mRNA?

A.mRNA degradation will be slowed
B.mRNA will be rapidly degraded
C.Translation will increase
D.Introns will not be spliced

16. Which of the following post-translational modifications can regulate protein activity?

A.Phosphorylation
B.Glycosylation
C.Ubiquitination
D.All of the above

17. Which of the following is NOT a stage of transcription?

A.Initiation
B.Elongation
C.Termination
D.Translation

18. Which statement best describes the difference between eukaryotic and prokaryotic translation?

A.Eukaryotic translation occurs in the cytoplasm, while prokaryotic translation occurs in the nucleus
B.Prokaryotic translation can occur simultaneously with transcription, while eukaryotic translation cannot
C.Eukaryotic translation requires ribosomes, while prokaryotic does not
D.There is no difference between the two

19. Fill in the blank: RNA polymerase adds nucleotides in the _____ direction.

A.5' to 3'
B.3' to 5'
C.5' to 5'
D.3' to 3'

20. What does the term 'peptidyl transferase' refer to?

A.The enzyme that adds amino acids to tRNA
B.The enzyme that catalyzes the formation of peptide bonds
C.The enzyme that synthesizes mRNA
D.The enzyme that degrades proteins

21. Which statement best describes exons?

A.They are non-coding sequences
B.They are spliced out of pre-mRNA
C.They are coding sequences that remain in mRNA
D.They are added during transcription

22. What is the purpose of chaperone proteins in post-translational modifications?

A.To aid in the degradation of misfolded proteins
B.To assist in the proper folding of newly synthesized proteins
C.To modify proteins by adding phosphate groups
D.To transport proteins to the Golgi apparatus

23. What initiates transcription in eukaryotic cells?

A.Binding of RNA polymerase to the terminator
B.Binding of RNA polymerase to the enhancer
C.Binding of RNA polymerase to the promoter
D.Binding of ribosomes to mRNA

24. Which codon is recognized as the start codon in eukaryotes?

A.AUG
B.UAA
C.UAC
D.GCG

25. Why is polyadenylation important for mRNA stability?

A.It helps in ribosomal binding
B.It prevents splicing errors
C.It protects mRNA from degradation
D.It initiates translation

26. What is the role of elongation factors during translation?

A.They initiate the binding of mRNA to ribosomes
B.They assist in the movement of the ribosome along the mRNA
C.They terminate the translation process
D.They modify proteins after translation

27. True or False: Transcription occurs in the cytoplasm of eukaryotic cells.

A.True
B.False
C.Only in mitochondria
D.Only in the nucleus

28. Which post-translational modification is commonly associated with signaling pathways?

A.Ubiquitination
B.Phosphorylation
C.Glycosylation
D.None of the above

29. What is the primary role of the 5' cap added during RNA processing?

A.It protects mRNA from degradation and assists in ribosome binding.
B.It facilitates the removal of introns from pre-mRNA.
C.It enhances the stability of the DNA template during transcription.
D.It serves as a binding site for transcription factors.

30. What happens during the termination stage of translation?

A.The ribosome assembles around the mRNA
B.A stop codon is recognized, and the polypeptide chain is released
C.A new amino acid is added to the growing chain
D.The ribosome moves to the next mRNA molecule

31. Which of the following statements about RNA splicing is FALSE?

A.Exons are joined together to form the final mRNA.
B.Introns are removed from the pre-mRNA sequence.
C.Splicing occurs in the nucleus before mRNA leaves.
D.Splicing is a process that adds extra coding sequences to mRNA.

32. What is the role of the E site in the ribosome?

A.It holds the tRNA carrying the growing polypeptide chain
B.It is where the newly arrived tRNA binds
C.It is where the tRNA exits after peptide bond formation
D.It catalyzes the formation of peptide bonds

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